Short-term buserelin administration induces apoptosis and morphological changes in adult rat testes

To investigate the effect of buserelin on gonadal structure and function in adult male
rats.
Methods: Twenty-four adult Wistar male rats were divided into three groups: two treated
groups and controls. The first and second treated groups received 300 (low dose) and 500 (high
dose) μg/kg buserelin, respectively, and the control group received normal saline. All groups
were treated subcutaneously for five days.
Results: The seminiferous tubular epithelial thickness was significant decreased in the treated
groups compared with those in the control. There was a significant increase in apoptotic cell
death in high dose treated group compared with low dose treated and control groups. No
significant difference in serum testosterone level was observed after one month in the three
groups.
Conclusion: Buserelin induces apoptotic cell death and decreased diameter and epithelium
thickness of seminiferous tubules in the adult rat testes.