Isolation of rapid growing mycobacteria from soil and water in Iran

Rahbar, M and Lameei, A and Babazadeh, H and Yavari, S.A (2010) Isolation of rapid growing mycobacteria from soil and water in Iran. African Journal of Biotechnology, 9 (24). pp. 3618-3621.

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Abstract

A total of 350 soil samples were collected from different part of Uremia city and suburbs. We used 3% sodium lauryl sulfate and 1% NaOH for decontamination of soil samples. Of 350 samples, mycobacteria were isolated from 65 (18.3%) specimens. Mycobacterium fortuitum with 18(5.14) strains yielded the highest frequency of isolation. The other isolates were: Mycobacterium peregrinum 11(3.14%), Mycobacterium flvescens 10 (2.85%), Mycobacterium chelonae 6 (1.71%), Mycobacterium mucogenicum 6(1.71%), Mycobacterium thermoresistable 4(1.14%), Mycobacterium abscessus 3 (0.85%), Mycobacterium neoaurum 2(0.57%), Mycobacterium smegmatis 2 (0.57%) and M. fortuitum third biovalant complex 3 (0.85%). The mean pH of soil was 7.89 ± 0.379 (max 8.5, min 7.5). Our data showed an abundant occurrence of mycobacteria in low pH (P value = 0001). We also collected 120 water samples from rivers, brooks and drinking water. Water samples decontaminated were by adding cetyl pyridinium chloride (CPC) to give final concentration of 0.05%. Mycobacteria isolated from 12 water samples. The predominant isolated species were M. fortuitum and Mycobacterium cheloni. The majority isolates were from brooks and surface waters.

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