INVESTIGATING PLATELET RICH PLASMA EFFECTS ON ADIPOSE-DERIVED STROMAL CELLSPROLIFERATION AND DIFFERENTIATION TO OSTEOBLAST

The cultured mesenchymal stem cells (MSCs) have been applied in many
clinical trials and there are some concerns about the culture conditions. One of them is related to the
use of fetal bovine serum (FBS). FBS is a xoengenic supplement withadverse effects which is used
widely in culture system and many attempts have been done to eliminate it. Platelet rich plasma (PRP)
is a candidate in the place of FBS.
Materials & Methods: Adipose-derived stromal cells (ADSCs) were isolated from liposuction tissues
and cultured in αMEM with 10% FBS. Cells of the third passage were used for the in vitro
experiments and characterized by flowcytometric analysis. PRP was obtained by two-step
centrifugation and PRP sample were activated by freezing method. Resazurinand mineralization
assays were used to determine the effects of PRP on cell proliferation and osteogenic differentiation.
Results: Treatment with PRP resulted in a statistically significant increase cell proliferation and
mineralization in comparison to negative control (P<0.001) and that of the 15% PRP group
significantly was higher than that of 10% PRP group (P<0.05). FBS showed the osteogenic
differentiation of ADSCs better than hPRP.
Conclusions: These findings indicate that PRP improves the proliferation and osteogenic
differentiation of ADSCs. Obviously, the biological effects of PRP were dose-dependent and can be a
useful solution for clinical applications in the place of FBS. However, for the clinical application of
PRP, more research is needed such as in vivo transplantation