Isolation, molecular identification and characterization of the culture conditions for extracellular uricase production by a new strain of Pseudomonas Sp.

Ghasemian, A and Moradpour, Z and Baniasad, M and Ghasemi, Y (2015) Isolation, molecular identification and characterization of the culture conditions for extracellular uricase production by a new strain of Pseudomonas Sp. Journal of Pure and Applied Microbiology, 9 (4). pp. 2813-2821.

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Text Isolation, molecular identification and statistical optimization of culture condition for a new extracellular cholesterol oxidase-producing strain using response surface methodology.pdf Download (341kB) | Preview

Abstract

The current work details the screening of about 100 isolates from various soil samples, from which 1 isolate was finally selected based on the productivity of cholesterol oxidase. Further biochemical identification tests and 16S rRNA gene sequencing identified this isolate as Streptomyces badius. A preliminary culture media optimization was carried out using the initial screening method of Plackett-Burman. Then, a Box- Behnken design was employed to investigate the optimum concentrations of medium components and interactive effects of main variables on cholesterol oxidase production. The regression analysis showed a significant coefficient of determination (R2) value (91 %), which was in close agreement ensuring a satisfactory adjustment of the proposed model. Maximal enzyme production (2.38 U/mL, i.e., approximately more than 100 % activity in the basal medium) was obtained at: temperature 35 °C; Tween 20 0.1 %; pH 6.5 and yeast extract 0.15 %. This two-stage statistical approach provided rapid identification and integration of key medium parameters for Streptomyces sp., resulting in high cholesterol oxidase production

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