DIAGNOSIS OF HELICOBACTER PYLORI INFECTION BY USING ELISA FOR DETECTION OF SERUM IGM, IGG AND IGA ANTIBODIES

Helicobacter pylori is a gram negative bacteria and is an etiologic agent of some gastrointestinal diseases such as chronic gastritis, gastric ulcers, lymphoma and adenocarcinoma. Many invasive and noninvasive methods for detection of this infection are available and among non invasive methods diagnostic value of specific IgM, IgG, and IgA antibodies by Eliza is one of the best methods. The aim of this study is to detect H.pylori antibodies for IgM, IgG and IgA by Elisa method.

Methods and Materials: Blood samples from 131 referred patients for detection of H.pylori antibodies were taken and serum was separated to detect of IgM, IgG and IgA antibodies. IgG was assessed by EIU units (<34 was negative and >42 was positive). Also for IgM and IgA antibodies reported by U/ml (<8 was negative and >12 was positive).

Results: One hundred and thirty one samples (78 female and 53 male) were tested for H.pylori IgM, IgG and IgA antibodies and showed 27.48%, 42% and 25.19% positive for IgM, IgG and IgA antibodies, respectively. The age group was between 20-40 years old for IgM, IgG and IgA antibodies 23.3% and 28.3% and 16.7 % positive respectively and for 41-80 years old IgM, IgG and IgA antibodies were 31%, 53.5% and 32.4% respectively. The above mentioned data showed infection was higher at 41 – 80 year-old patients.

Conclusion: Simple methods for detection of H.pylori antibodies are available commercially and have sensitivity and specificity for H.pylori diseases. IgM, IgG and IgA have diagnostic importance i.e IgG for prognosis and IgM for detection of acut infection of diseases, therefore regarding acceptable sensitivity and specificity, ease of work with Elisa, being economical and non invasive, it can be employed in diagnosis of H.pylori infections.