Short-term buserelin administration induces apoptosis and morphological changes in adult rat testes

To investgate the effect of buserelin on gonadal structure and functon in adult male
rats.
Methods: Twenty-four adult Wistar male rats were divided into three groups: two treated
groups and controls. The frst and second treated groups received 300 (low dose) and 500 (high
dose) µg/kg buserelin, respectvely, and the control group received normal saline. All groups
were treated subcutaneously for fve days.
Results: The seminiferous tubular epithelial thickness was signifcant decreased in the treated
groups compared with those in the control. There was a signifcant increase in apoptotc cell
death in high dose treated group compared with low dose treated and control groups. No
signifcant difference in serum testosterone level was observed afer one month in the three
groups.
Conclusion: Buserelin induces apoptotc cell death and decreased diameter and epithelium
thickness of seminiferous tubules in the adult rat testes.