Protective effects of intraperitoneal administration of nimodipine on 3 ischemia – reperfusion injury in ovaries: Histological and biochemical 4 assessments in a rat model

Behroozi Lak, T and Zarei, L and Moloody Tapeh, M and Mohammadi, R and Farhadmand, N (2016) Protective effects of intraperitoneal administration of nimodipine on 3 ischemia – reperfusion injury in ovaries: Histological and biochemical 4 assessments in a rat model. Journal of pediatric. pp. 1-8.

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Abstract

UNCORRECTED PROOF 1 2 Protective effects of intraperitoneal administration of nimodipine on 3 ischemia – reperfusion injury in ovaries: Histological and biochemical 4 assessments in a rat model 5 Q1 Tahereh Behroozi-Lak a , Leila Zarei b , ⁎ , Mones Moloody – Tapeh c ,NeginFarhad d , Rahim Mohammadi e 6 a Reproductive Health Research Center, Department of Infertility, Urmia University of Medical Sciences, Urmia, Iran 7 b Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran 8 c Department of Nursing and Midwifery, Urmia Branch, Islamic Azad University, Urmia, Iran 9 d Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran 10 e Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran abstract 11 article info 12 Article history: 13 Received 5 June 2016 14 Received in revised form 31 August 2016 15 Accepted 29 September 2016 16 Available online xxxx 17 Key words: 18 Ischemia – reperfusion 19 Nimodipine 20 Intraperitoneal 21 Ovary 22 Biochemical 23 Histopathology 24 Rat 25 Purpose: Ovarian torsion must be diagnosed and treated as much early as possible. The aim of the present study 26 wastoinvestigateeffectsofintraperitone al administration of nimodipine on ischemia – reperfusion injury in ovaries. 27 Methods: Thirty healthy male Wistar rats weighing approxima tely 250 g were randomized into six experimental 28 groups (n = 5): Group Sham: The rats underwent only laparotomy. Group I: A 3-h ischemia only. Group I/R: 29 A 3-h ischemia and a 3-h reperfusion. Group I/Nimodipine: A 3-h ischemia only and 1 mg/kg intraperitoneal 30 administration of nimodipine 2.5 h after induction of ischemia. Group I/R/Nimodipine: A 3-h ischemia, a 3-h 31 reperfusion and 1 mg/kg intraperitoneal administration of nimodipine 2.5 h after induction of ischemia. 32 Results: Nimodipine treated animals showed signi fi cantly ameliorated development of ischemia and reperfusion 33 tissue injury compared to those of other groups ( P b 0.05). The signi fi cant higher values of SOD, tGSH, GPO, 34 GSHRd and GST were observed in I/R/Nimodipine animals compared to those of other groups ( P b 0.05). The 35 damage indicators (NOS, MDA, MPO and DNA damage level) were signi fi cantly lower in I/R/Nimodipine animal 36 compared to those of other groups ( P b 0.05). 37 Conclusions: Intraperitoneal administration of nimodipine could be helpful in minimizing ischemia – reperfusion 38 injury in ovarian tissue exposed to ischemia

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