Protective effects of intraperitoneal administration of nimodipine on 3 ischemia – reperfusion injury in ovaries: Histological and biochemical 4 assessments in a rat model

UNCORRECTED PROOF
1
2
Protective effects of intraperitoneal administration of nimodipine on
3
ischemia
–
reperfusion injury in ovaries: Histological and biochemical
4
assessments in a rat model
5
Q1
Tahereh Behroozi-Lak
a
, Leila Zarei
b
,
⁎
, Mones Moloody
–
Tapeh
c
,NeginFarhad
d
, Rahim Mohammadi
e
6
a
Reproductive Health Research Center, Department of Infertility, Urmia University of Medical Sciences, Urmia, Iran
7
b
Solid Tumor Research Center, Urmia University of Medical Sciences, Urmia, Iran
8
c
Department of Nursing and Midwifery, Urmia Branch, Islamic Azad University, Urmia, Iran
9
d
Student Research Committee, Urmia University of Medical Sciences, Urmia, Iran
10
e
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
abstract
11
article info
12
Article history:
13
Received 5 June 2016
14
Received in revised form 31 August 2016
15
Accepted 29 September 2016
16
Available online xxxx
17
Key words:
18
Ischemia
–
reperfusion
19
Nimodipine
20
Intraperitoneal
21
Ovary
22
Biochemical
23
Histopathology
24
Rat
25
Purpose:
Ovarian torsion must be diagnosed and treated as much early as possible. The aim of the present study
26
wastoinvestigateeffectsofintraperitone
al administration of nimodipine on ischemia
–
reperfusion injury in ovaries.
27
Methods:
Thirty healthy male Wistar rats weighing approxima
tely 250 g were randomized into six experimental
28
groups (n = 5): Group Sham: The rats underwent only laparotomy. Group I: A 3-h ischemia only. Group I/R:
29
A 3-h ischemia and a 3-h reperfusion. Group I/Nimodipine: A 3-h ischemia only and 1 mg/kg intraperitoneal
30
administration of nimodipine 2.5 h after induction of ischemia. Group I/R/Nimodipine: A 3-h ischemia, a 3-h
31
reperfusion and 1 mg/kg intraperitoneal administration of nimodipine 2.5 h after induction of ischemia.
32
Results:
Nimodipine treated animals showed signi
fi
cantly ameliorated development of ischemia and reperfusion
33
tissue injury compared to those of other groups (
P
b
0.05). The signi
fi
cant higher values of SOD, tGSH, GPO,
34
GSHRd and GST were observed in I/R/Nimodipine animals compared to those of other groups (
P
b
0.05). The
35
damage indicators (NOS, MDA, MPO and DNA damage level) were signi
fi
cantly lower in I/R/Nimodipine animal
36
compared to those of other groups (
P
b
0.05).
37
Conclusions:
Intraperitoneal administration of nimodipine could be helpful in minimizing ischemia
–
reperfusion
38
injury in ovarian tissue exposed to ischemia