Zinc attenuates ethanol-induced Sertoli cell toxicity and apoptosis through caspase-3 mediated pathways

Pourhassanali, N and Roshan Milani, SH and Motazakker, M and Bagheri, M and Kheradmand, F and Saboory, E (2016) Zinc attenuates ethanol-induced Sertoli cell toxicity and apoptosis through caspase-3 mediated pathways. Reproductive Toxicology. pp. 1-8.

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Abstract

Ethanol enhances apoptosis in testicular germ cells. Zinc reduces ethanol-induced apoptosis of somatic cells through inhibition of caspase-mediated pathways. Little is known about the effects of ethanol on Sertoli cells and the effects of Zinc on ethanol-induced testicular injury. The hypothesis tested was that ethanol enhances apoptosis of Sertoli cells through up-regulation of caspase-3 and Zinc inhibits ethanol- induced effects. Cultured Sertoli cells (TM4) were exposed to ethanol (160 mM), Zinc (8 � M) and Zinc prior to ethanol for duration of 24 or 48 h and their effects on TM4 cell viability was then investigated by MTT assay. Caspase-3 mRNA expression was also investigated using real-time RT-PCR. Cell viability decreased and caspase-3 mRNA expresstion increased in cells exposed to ethanol, while exposure to Zinc showed opposite effects. Pretreatment with Zinc recovered ethanol-induced anti-proliferative effects and over-expression of caspase-3. Zinc reduced ethanol-induced Sertoli cell toxicity and apoptosis via caspase-3 mediated pathways

Item Type: Article
Uncontrolled Keywords: Apoptosis Caspase-3 Ethanol Zinc Sertoli cell
Subjects: R Medicine > R Medicine (General)
Depositing User: Unnamed user with email gholipour.s@umsu.ac.ir
Date Deposited: 09 Oct 2018 07:09
Last Modified: 27 Jan 2019 05:22
URI: http://eprints.umsu.ac.ir/id/eprint/5187

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