Role of Helicobacter pylori on cancer of human adipose-derived mesenchymal stem cells and metastasis of tumor cells-an in vitro study

Recent studies have shown that Helicobacter pylori
has a special role in tropism of mesenchymal stem cells
(MSCs) towards gastric tissues. This study aimed to find the
effects of H. pylori on human adipose-derived mesenchymal
stem cells (hA-MSCs) transforming toward cancer cells and
also metastasis of tumor cells by synergic effects of H. pylori
and gastric epithelial cells (AGS) on MSCs. The expressions
of p53, bcl-2, MMP-2, and MMP-9 were examined in hAMSCs by qRT-PCR technique. Our results demonstrated that
H. pylori tries to improve the hA-MSCs carcinogenic activities by overexpression of bcl2 gene as an anti-apoptosis agent
against the p53 gene expression as main apoptosis agent. In
addition, it showed that H. pylori effects in metastatic activities of hA-MSCs by upregulation of related genes in this process. Perhaps, when hA-MSCs are attracted toward H. pylori
chronic or ulcer infected tissues for their tissue healing function, they will be trapped under special gastric microenvironment. We demonstrated the direct and synergic effects of
H. pylori in hA-MSCs through alteration of related genes involved in carcinogenesis processes. Hence, understanding of
H. pylori-induced molecular pathogenesis could be a powerful
strategy not only in identifying the origin and initiation of
gastric cancer but also in the treatment of related disease and
modification of stem cell therapy methods in the future