Determination of the relationship between the severity of Behcet's disease and the expression and methylation of IL-10, IL-6 and IL-8 genes

Abolhasani, S and Gholizadeh Ghaleh Aziz, SH and Khabbazi, A.R and Alipour, SH (2018) Determination of the relationship between the severity of Behcet's disease and the expression and methylation of IL-10, IL-6 and IL-8 genes. International Journal of Research in Applied and Basic Medical Sciences, 4 (1). pp. 6-14.

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Abstract

The immune system interference in Behcet's disease (BD) is typically cleared by Th1 as well as Th17 pathway - mediated inflammatory process.Among these, Interleukin-10 and 6, 8 as cytokines involved in inflammation have played a major role in autoimmune diseases. The aim of this study was to investigate the relationship between the severity of Behcet's disease with gene expression and methylation of IL-10, IL-6 and IL -8 genes. Materials and Methods: Since this project had been investigated in a large study, we examined the association of IL10, IL6 and IL8 genes with the severity of BD. Accordingly, all analyses in this paper are limited to people with Behcet's. In this study, 26 patients are with severe BD and 21 with no severe BD. So, we examined Relationship between severity of BD disease and expression and methylation in these genes through appropriate statistical methods. Results: The mean expression rate of IL6 and IL8 genes in positive subjects with severe BD is higher than that of the ones with no severe BD; however, this is opposite in IL10. In addition, the methylation level of IL6 and IL10 genes in no severe BD subjects is greater than that of those with severe BD, however it is opposite in IL8.The difference between the expressions of IL10 in two groups and the difference between the methylation levels of IL8 and IL10 genes were reported to be significant (P <0.05). Conclusion: In general, our results did not suggest any correlation between gene expressions of IL-10, Il-6, and IL-8. However, expression and methylation of these genes in the studied groups were difference in terms of disease severity

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