Nitric oxide and nicotine amide adenine dinucleotide phosphate oxidase activity before and after renal transplantation

Renal transplantation has been considered as the best therapeutic strategy for end stage renal disease (ESRD).
Oxidative stress induced by the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and nitric oxide has key
role in the pathogenesis of ESRD. Therefore, in this study, the propose of this research was to investigate the enzymatic activity of
NADPH oxidase (NOX), as well as nitric oxide (NO) levels before and after renal transplantation,
Materials and Methods: Twenty-five patients with ESRD and renal transplantation with age range of 20-60 years was enrolled in this
study. Serum samples were collected for measurement of NADPH oxidase and NO levels, before, one hour and 14 days after
transplantation, by ELISA technique using a commercial kit. Serum levels of urea and creatinine was also evaluated.
Results: The serum urea and creatinine levels significantly decreased after transplantation (p<0.05). research indicated that the serum
levels of NADPH oxidase and NO was increased in the three times, one hour and 14 days after transplantation, but this increase was
not statistically significant.In addition, NO and NADPH oxidase showed a significant correlation.
Conclusion: This study showed that the serum levels of NADPH oxidase and NO was not significantly changed before and after renal
transplantation. In addition, NO had no diagnostic value in renal transplant patients who did not have acute rejection