Nitric oxide and nicotine amide adenine dinucleotide phosphate oxidase activity before and after renal transplantation

Khadem Ansari, M.H and Rasmi, Y and Kheyri, N and Taghizadeh Afshari, A (2018) Nitric oxide and nicotine amide adenine dinucleotide phosphate oxidase activity before and after renal transplantation. International Journal of Research in Applied and Basic Medical Sciences, 4 (2). pp. 74-80.

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Abstract

Renal transplantation has been considered as the best therapeutic strategy for end stage renal disease (ESRD). Oxidative stress induced by the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and nitric oxide has key role in the pathogenesis of ESRD. Therefore, in this study, the propose of this research was to investigate the enzymatic activity of NADPH oxidase (NOX), as well as nitric oxide (NO) levels before and after renal transplantation, Materials and Methods: Twenty-five patients with ESRD and renal transplantation with age range of 20-60 years was enrolled in this study. Serum samples were collected for measurement of NADPH oxidase and NO levels, before, one hour and 14 days after transplantation, by ELISA technique using a commercial kit. Serum levels of urea and creatinine was also evaluated. Results: The serum urea and creatinine levels significantly decreased after transplantation (p<0.05). research indicated that the serum levels of NADPH oxidase and NO was increased in the three times, one hour and 14 days after transplantation, but this increase was not statistically significant.In addition, NO and NADPH oxidase showed a significant correlation. Conclusion: This study showed that the serum levels of NADPH oxidase and NO was not significantly changed before and after renal transplantation. In addition, NO had no diagnostic value in renal transplant patients who did not have acute rejection

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