EVALUATION OF ANTIOXIDANT AND ANTIFUNGAL PROPERTIES OF NEW DERIVATIVES OF PERAZOLO [3,4-D]PYRIMIDINE

Heterocyclic compounds are found be abundant in nature, these compounds have
many biological properties, and many drugs also contain heterocyclic rings. In this study, antioxidant
and anti-fungal properties of the seven pyrazolo[3,4-d]pyrimidine compounds has been studied that
synthesized in Iran and their good antibacterial properties have been reported
Materials & Methods: Antioxidant properties were evaluated using the DPPH (2,2-diphenyl-1-
picrylhydrazyl) method. Antifungal property was evaluated using CLSI (Clinical & Laboratory
Standards Institut) standard based on inhibition zone diameter, minimum inhibitory concentration and
minimum fungicidal concentration and compared with anti-fungal drugs including Ketoconazole,
Nystatin and Tolnaftate.
Results: Concentrations of 25, 50, 75 and 100 μg/mL of the derivatives were prepared in methanol, after
performing an antioxidant test with DPPH, the percentage of inhibition was reported, in addition, the
IC50 was calculated for each compound, and finally the test for ascorbic acid was done and the results
were compared. In the study of antifungal activity, after the preparation of specific concentrations of
derivatives, the results of inhibition zone diameter, minimum inhibitory concentration and minimum
fungicidal concentration were reported and the tests were performed for Ketoconazole, Nystatin and
Tolnaftate drugs and finally the results of the derivatives with the drugs were compared.
Conclusion: In this study, new synthetic compounds of pyrazolo[3,4-d]pyrimidine showed antioxidant
activity. In addition, these compounds have strong antifungal properties, and even some derivatives have
better results and effective than anti-fungal commercial drugs