Abedi Gaballu, F and Dehghan, G.R and Mansoori, B (2019) EVALUATION OF ANTI-CANCER ACTIVITY OF COVALENTLY CONJUGATED METHOTREXATE TO POLYAMIDOAMINE GENERATION 4 DENDRIMER ON MCF-7 CANCER CELLS: AN EXPERIMENTAL STUDY. The Journal of Urmia University of Medical Sciences, 30 (5). pp. 381-392.
|
Text
6 Abedi ghilou A-10-4094-2.pdf Download (850kB) | Preview |
Abstract
Poly (amidoamine) dendrimer (PAMAM) is highly macromolecular at nanosize with widely active amine groups on the surface that allows it to attach to the anti-cancer drugs such as Methotrexate (MTX). This study aimed to synthesize and characterize PAMAM-MTX (dendrimer- MTX) complex, then to evaluate the cytotoxic effect of the synthesized complex onMCF-7 cancer cells. Materials & Methods: To perform this experimental study, initially, MTX was conjugated to the G4 dendrimer using Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), then it was characterized by Fourier-transform infrared (FTIR) and dynamic light scattering (DLS). Quantitative cellular uptake of nanoparticles was carried out using flow cytometry. The methylthiazol tetrazolium (MTT) and 4′, 6- Diamidino-2-phenylindole dihydrochloride (DAPI) staining assays were used for evaluating the ability of dendrimer-MTX and MTX in inducing cell cytotoxicity and apoptosis on MCF-7 cancer cells, respectively. Results: The results of this study illustrated that the dendrimer-MTX complex had optimal size (30 ± 7.29 nm) and zeta potential (5.35 ± 4.37 mV). Flow cytometry analysis revealed that the cellular uptake was increased (18%) after conjugating MTX on the dendrimer compared to bare dendrimer. Interestingly, cell cytotoxicity and DAPI staining results displayed lower cell cytotoxicity and apoptosis for MCF-7 cells that were treated with dendrimer-MTX compared to free MTX. Conclusion: The obtained data indicated that dendrimer-MTX complex had a higher cellular uptake, but cell cytotoxicity and apoptosis were lower than free MTX.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Methotrexate, dendrimer, apoptosis, cytotoxicity, cellular uptake. Address: Department of Biology, Faculty of Natural Sciences, Uni |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
| Date Deposited: | 07 Sep 2019 04:26 |
| Last Modified: | 07 Sep 2019 04:26 |
| URI: | http://eprints.umsu.ac.ir/id/eprint/5641 |
Actions (login required)
 |
View Item |
