EVALUATION OF HEPATOKINE AND LIVER ENZYMES CHANGES IN OBESE RATS WITH THE HIGH-FAT DIET TO DIFFERENT TRAINING MODALITIES: AN EXPERIMENTAL STUDY

Exercise with modulation of hepatokine counteracts pathological abnormalities
of the liver due to fat accumulation. Therefore, the purpose of this study was to investigate the effect of
high-intensity continuous training (HICT), high-intensity interval training (HIIT), and moderate-
intensity continuous training (MICT) on Fetuin-A, FGF-21 and liver enzymes of the obese rats with
high-fat diet.
Materials & Methods: A total of 25 maleWistar rats were divided into 5 groups: 1) healthy control; 2)
high-Fat Diet (HFD) control; 3) HICT; 4) HIIT and 5) MICT. Except for the healthy control group, all
rats were subjected to the high-fat diet for 12 weeks and then the training rats were trained for 12 weeks,
5 sessions a week. Fetuin-A and FGF-21 values were measured by the ELISA method. Data were
analyzed by dependent t-test and one-way ANOVA at a significance level of P≤0.05.
Results: The results of the ANOVA test showed that there is a significant difference between groups in
Fetuin-A (P = 0.001 and F=7.670) and FGF-21 (P= 0.010 and F=4.394). It was also found that different
training modalities had a different effect on hepatokine (MICT: a significant reduce in Fetuin-A and
HICT: a significant increase in FGF-21) and had the same effect on ALT and AST. The HFD control
significantly increased liver enzymes (P= 0.001). However, all the exercise types significantly decreased
these enzymes (for all groups, P= 0.001).
Conclusion: MICT exercises are more capable of controlling liver negative hepatokine such as Fetuin-
A that prevent insulin resistance. However highintensity exercises such as HICT can increase the
positive factors, such as FGF-21, that modifies the activity of the liver enzymes. As a result, it seems
that obese subjects can use moderate to high-intensity exercises to prevent fatty liver.