THE EFFECT OF NICOTINAMIDE ON SERUM INFLAMMATORY CYTOKINE LEVELS, FASTING BLOOD SUGAR, SERUM C-PEPTIDE, AND INSULIN ON EXPERIMENTAL AUTOIMMUNE DIABETES IN C57BL/6 MICE

Jafari Khataylou, Y and Rasouli, M (2019) THE EFFECT OF NICOTINAMIDE ON SERUM INFLAMMATORY CYTOKINE LEVELS, FASTING BLOOD SUGAR, SERUM C-PEPTIDE, AND INSULIN ON EXPERIMENTAL AUTOIMMUNE DIABETES IN C57BL/6 MICE. Urmia Medical Journal, Vol. 30(9), December 2019, 30 (9). pp. 720-730.

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Abstract

Destruction of insulin-producing beta-cells by T cells causes type-1 diabetes (T1D). Since nicotinamide has cytoprotective and anti-apoptotic effects and has been shown to play a useful role in the prevention of T1D, in this study, the effect of nicotinamide on T1D in C57BL / 6 mice was studied. Materials&Methods: Thirty maleC57BL/6 inbred strain mice were randomly divided into three groups and in two groups diabetes was induced by streptozotocin (Diabetic control and therapeutic groups). In the therapeutic group, nicotinide was injected subcutaneously 0.5 mg/g body Wt/day for 40 days, 20 days before Stz injection up to 20 days after first Stz injection and then, on the 41st day after the first injection of the drug, the levels of fasting blood glucose (FBS), C-peptide, serum insulin, and inflammatory cytokines Il-1β, TNF-α and IFN-γ were studied. Results: The results showed that nicotinamide decreased levels of IL-1β, TNF-α, IFN-γ and increased serum insulin and C-peptide levels, which was statistically significant (p <0.05). Conclusion: Nicotinamide reduced apoptosis and beta-cell destruction by reducing the number of inflammatory cytokines, and lessened the severity of the disease.

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