kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite

Mirahmadi, H and Gholizadeh, S and Raeghi, S and Sadat Roointan, E and Rezaee, N and Mehravaran, A (2019) kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite. Journal of KermanUniversity ofMedical Sciences, 26 (1). pp. 1-11.

Preview

Text 1530.pdf Download (2MB) | Preview

Abstract

CutaneousLeishmaniasis, is endemicallyobserved in different parts of Iran in two forms of anthroponotic and zoonotic. The identification of both species and the type of disease are beneficial for treatment and prevention.Microscopic identificationofLeishmania species has not provided promising efficacy. The aim of this studywas to determine the Leishmania species that are responsible for cutaneous leishmaniasis in Zahedan/ Iran by using PCR and PCR-RFLP techniques. Direct smears were obtained from cutaneous leishmaniasis suspected individualswith low parasitemia in cutaneous lesions referred to Zahedan health centers. Eventually, the DNA was extracted from smears usingDNA extractionkit.PCRwas used to amplify bothLeishmania kinetoplastic DNA (kDNA) and ITS1 locus of ribosomal DNA. Additionally, PCR-RFLP on ITS1 productswas conducted to determine parasite species. PCR-RFLP test (detecting ITS1 locus) on all positive samples inmicroscopic analysis led to the identification of Leishmaniamajor in 52 samples (54.7%), and 43 caseswere detected to have Leishmania tropica (45.3%). On the other hand, kDNA-PCR results indicated a frequency of 68 (55.7%) for L.major and 54 (44.3%) for L. tropica. Due to thehigh frequencyof kDNA in parasiticgenome,PCR-kDNAcompared to PCR-RFLP shows a higher efficiency and accuracy not only in identifying infection, but also in determining parasite species, especially among the patients with fewer lesions. This study also indicates that both L. tropica and L. major could be found in Zahedan, with a greater tropical leishmaniasis endemicity.

Actions (login required)

View Item