The effect of Silver nanoparticles on biofilm production of vancomycin resistant Staphylococcus aureus

The increasing rate of vancomycin resistant Staphylococcus aureus (VRSA) with biofilm formation may become
a new threat to humans. In such cases, finding an effective treatment strategy such as using Nanotechnology (Nano- drugs) to deal with
these types of infections may be promising. This study aimed to investigate the inhibitory effects of silver nanoparticles (SNPs) on
biofilm formation of VRSAs.
Materials and Methods: Clinical S. aureus isolates were identified to the species level by conventional methods, and their identities
were later confirmed by PCR. Following the determination of susceptibility patterns of the isolates; all the screened S.aureus isolates
have been assessed regarding their susceptibility to vancomycin. Detection of vanA gene and determination of minimum inhibitory
concentrations (MICs) of VRSAs were carried out using PCR and Etest methods, respectively. The biofilm production was assessed
on all VRSA isolates in the presence/absence of SNPs using micro-titer plate method.
Results: In total, 11 (6.21%) VRSAs were identified among 177 S. aureus clinical isolates. These isolates were included in the biofilm
production assay. All of the VRSAs were multidrug resistance and biofilm producers. The inhibitory effect of SNPs in concentration
of 250 µg/ml on biofilm formation of VRSA isolates was significant (Pv = 0.01).
Conclusion: Based on our findings, SNPs can prevent biofilm formation of VRSAs and applying of these nanoparticles may prohibit
from the persistence and colonization of such resistant isolates.