NEW METHOD FOR DETERMINATION OF AMIODARONE IN SERUM WITH DISPERSIVE LIQUID-LIQUID MICROEXTRACTION BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH EXPERIMENTAL METHOD

Amiodarone is an anti-arthritis drug (III) used to treat various types of
arrhythmias. Determining the concentration of this drug in the blood of patients is important for the
administration of this drug and its interaction with other drugs (due to its high interaction with other
drugs). It is necessary to use precision instruments such as HPLC with expensive detectors, but the
analysis is often time-consuming and the methods of extraction have a high cost. Therefore, the liquid-
liquid extraction method for rapid and accurate measurement of this medication was used in serum.
Materials & Methods: The liquid-liquid extraction method was used with a variety of solvent extraction
and different types of solvent. After selecting suitable solvents and optimizing various experimental
parameters, this method was used to extract and measure the concentration of amiodarone in Blood
serum.
Results: The results showed that carbon tetrachloride is suitable as a solvent extraction and acetonitrile
is suitable as a solvent, and their optimal volume is 75 and 200 μl, respectively, and the optimal volume
of the serum is 100 μl and the appropriate pH is in the two extraction steps 1 and 6. Mean calibration
curve of Amiodarone is linear in the range of 0.05-0.0 μg / ml and has a relative standard deviation of
3.79%.
Conclusion: The results indicate that this method is suitable for measuring amiodarone in blood serum
and reduces the time and cost of the analysis to a satisfactory level and has a good repeatability. With
this method, it is easy to measure the effect of serum tissue in measuring amiodarone