COMPARISON OF ANTI-CANCER EFFECTS OF HYDROALCOHOLIC EXTRACT OF SYZYGIUM AROMATICUM AND UTRICA DIOICA ON BREAST CANCER CELLS (MCF-7) AND NORMAL CELLS (HUVEC)

Soltani, L and Moarrefzadeh, N and Mohebbi, Z and Darbemamieh, M (2021) COMPARISON OF ANTI-CANCER EFFECTS OF HYDROALCOHOLIC EXTRACT OF SYZYGIUM AROMATICUM AND UTRICA DIOICA ON BREAST CANCER CELLS (MCF-7) AND NORMAL CELLS (HUVEC). Studies in Medical Sciences, 32 (3). pp. 175-186.

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Abstract

One of the leading causes of death in the world is cancer. In order to find cures with fewer side effects and on the other hand drug resistance problems that exist in relation to the treatment of cancer patients, attention to plant-derived products has greatly increased. The aim of this study was to compare the cytotoxicity of hydro-alcoholic extracts of nettle and clove on cancer and normal cells. Materials & Methods: Hydro-alcoholic extracts of nettle and clove were prepared in different concentrations (25, 100, 400, and 1200 μg / ml) then added to culture medium of breast cancer cells (MCF-7) and normal cells (Huvec) and incubated for 24 or 72 h. At the end of incubation, cytotoxicity was assessed by MTT assay and apoptosis by acridine orange-ethidium-bromide dual staining. The obtained data were analyzed using SPSS software and Duncan's multiple comparisons. Results: The addition of hydroalcoholic extracts of both nettle and clove at the highest used concentration (1200 μg/ml) after 24 and 72 hreduced cell proliferation significantly compared to other treatment and control groups (p<0.05). The addition of the highest concentration of both hydro-alcoholic extracts of clove and nettle to normal cells reduced cell proliferation significantly compared to other treatment groups (p<0.05). Conclusion: The results of this study showed that the hydro-alcoholic extracts of nettle and clove inhibit the proliferation of breast cancer cells (MCF-7).

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