INVESTIGATION OF C49620T POLYMORPHISM IN ABCC8 GENE IN PATIENTS WITH TYPE 2 DIABETES IN THE EAST AZERBAIJAN

Type 2 diabetes is a chronic disease that threatens public health. The disease is
on the rise with high complications worldwide. The C49620T variant of the ABCC8 gene is the most
common polymorphism associated with type 2 diabetes. The aim of this study was to investigate the
association of C49620T polymorphisms of ABCC8 gene with type 2 diabetes in the East Azerbaijan
population.
Materials & Methods: A total of 100 blood samples was collected from patients with diabetes and 100
blood samples were also collected from healthy individuals. After DNA extraction from all samples and
electrophoresis, the samples were subjected to specific primers, PCR, and electrophoresis. Genotyping
was performed using PCR-RFLP method. Finally, PCR products of Pst1 restriction enzyme were treated
and electrophoresed again and target polymorphism was observed.
Results: The results showed that the frequency distribution of TT, CC, and CT genotypes in the patient
and healthy groups was 26, 52, 22% and 53, 17 and 30%, respectively. The statistical comparison of the
frequency of CT genotype between healthy and diabetic individuals was not significant (p> 0.05), but
there was a significant difference between the frequency of TT and CC in healthy and diabetic
individuals (p <0.05). It is noteworthy that the percentage of T allele was 37% and 68% in healthy and
diabetic individuals and 63% and 32% in healthy and diabetic individuals, respectively.
Conclusion: The comparisons in this study indicate that there may be a relationship between C49620T
polymorphism in the intron of ABCC8 gene and diabetes mellitus. Therefore, more extensive studies
can lead to more accurate results and examine the susceptibility to diabetes in different populations