INVESTIGATION OF C49620T POLYMORPHISM IN ABCC8 GENE IN PATIENTS WITH TYPE 2 DIABETES IN THE EAST AZERBAIJAN

Joudi Shahabad, SH and Ghojaie, M and Hosseinpour Shordarag, F and Soltanzade, H (2021) INVESTIGATION OF C49620T POLYMORPHISM IN ABCC8 GENE IN PATIENTS WITH TYPE 2 DIABETES IN THE EAST AZERBAIJAN. Studies in Medical Sciences, 32 (3). pp. 187-195.

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Abstract

Type 2 diabetes is a chronic disease that threatens public health. The disease is on the rise with high complications worldwide. The C49620T variant of the ABCC8 gene is the most common polymorphism associated with type 2 diabetes. The aim of this study was to investigate the association of C49620T polymorphisms of ABCC8 gene with type 2 diabetes in the East Azerbaijan population. Materials & Methods: A total of 100 blood samples was collected from patients with diabetes and 100 blood samples were also collected from healthy individuals. After DNA extraction from all samples and electrophoresis, the samples were subjected to specific primers, PCR, and electrophoresis. Genotyping was performed using PCR-RFLP method. Finally, PCR products of Pst1 restriction enzyme were treated and electrophoresed again and target polymorphism was observed. Results: The results showed that the frequency distribution of TT, CC, and CT genotypes in the patient and healthy groups was 26, 52, 22% and 53, 17 and 30%, respectively. The statistical comparison of the frequency of CT genotype between healthy and diabetic individuals was not significant (p> 0.05), but there was a significant difference between the frequency of TT and CC in healthy and diabetic individuals (p <0.05). It is noteworthy that the percentage of T allele was 37% and 68% in healthy and diabetic individuals and 63% and 32% in healthy and diabetic individuals, respectively. Conclusion: The comparisons in this study indicate that there may be a relationship between C49620T polymorphism in the intron of ABCC8 gene and diabetes mellitus. Therefore, more extensive studies can lead to more accurate results and examine the susceptibility to diabetes in different populations

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