THE EFFECT OF TWO MONTHS OF CHIA SUPPLEMENTATION ON INFLAMMATORY AND ANTI-INFLAMMATORY RESPONSES IN WISTAR DIABETIC MALE RATS

Increased inflammation is a consequence of diabetes. Accordingly, the use of
herbal medicine as a solution has been suggested. The objective of this study was to evaluate the effect
of two months of chia supplementation in conjunction with inflammatory and anti-inflammatory
responses in male Wistar diabetic rats.
Materials & Methods: 36 diabetic rats were randomly assigned to 3 equal groups (control group,
diabetes group, diabetes + chia group) and were subjected to 8 weeks of supplementation. Interleukin-1
and interleukin-13 were measured in soleus muscle tissue to examine the inflammatory and antiinflammatory
indicators. ANOVA and Tukey's post hoc test were used for data analysis.
Results: Although chia supplementation had aggravating effects on IL-1ß compared with the diabetic
group, this effect was not statistically significant (p = 0.99). Also, IL-13 protein expression increased
significantly in the diabetic control group by about 51% (p = 0.001), and in the diabetic group with chia
supplementation by about 50% (p= 0.001) compared to the healthy control group. There was no
significant difference between the two groups.
Conclusion: It seems that two months of chia supplementation with this dose and period of consumption
could not have a positive effect on anti-inflammatory factors in male diabetic rats. Confirmation of this
finding requires further research in this area