INVESTIGATION OF PARAOXONASE1 ACTIVITY RATIO TO ARYL ESTERASE OF PARAOXONASE 1 ENZYME AND FREQUENCY OF NACETHYLTRANSFERASE- 2 ENZYME POLYMORPHISMS IN PATIENTS WITH PARKINSON'S DISEASE: A CASE-CONTROL STUDY

Parkinson's disease is the second most commonly diagnosed neurological disease
after Alzheimer's disease. Paraoxonase 1 enzyme is an antioxidant enzyme with two paraoxonase and
aryl esterase activity, and the change in activity and concentration of this enzyme can contribute to
Parkinson's disease. The N-acetyltransferase enzyme plays an important role in the detoxification of
organophosphates, in which the N-acetyltransferase 2 enzyme plays an important role. The aim of this
study was to evaluate the paraoxonase activity ratio to aryl esterase activity of paraoxonase enzyme and
the frequency of N-acetyltransferase 2 enzyme polymorphisms in patients with Parkinson's disease.
Materials & Methods: In this case-control study, 80 cases (40 patients with Parkinson's disease and 40
control subjects) were studied. N-acetyltransferase-2 polymorphisms by the PCR-RFLP method were
investigated using the relevant bound restriction enzymes. The serum level of paraoxonase 1 was
determined by ELISA and enzyme activity of paraoxonase 1 was measured with spectrophotometric.
Results: The ratio of paraoxonase to Aryl esterase did not show any significant difference between the
two groups (p >0.05). Significant differences were observed in the frequency of m1 polymorphism
genotypes between control and patient groups (p <0.05). However, the prevalence of polymorphism
genotypes m1 and m2 between the two groups did not show a significant difference (p> 0.05).
Conclusion: The results of this study showed that three N-acetyltransferase 2 polymorphisms, m1
polymorphism, as well as a decrease in serum levels and paraoxonase 1 activity, may contribute to the
pathogenesis and etiology of Parkinson's disease