Abdolrahman Shaban, Shadi and Rezaie, Jafar and Nejati, Vahid (2022) Exosomes Derived from Senescent Endothelial Cells Contain Distinct Pro‑angiogenic miRNAs and Proteins. Cardiovascular Toxicology.
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Abstract
Exosomes from senescence cells play pivotal roles in endothelium dysfunction. We investigated the exosomal angiogenic cargo of endothelial cells (ECs) in a model of senescence in vitro. After inducing aging by H 2 O 2 , the expression of P53, P21, and P16 was investigated by western blotting, while the expression of FMR1, miR-21, and miR-126 were measured by real-time PCR (q-PCR). Oil Red O dye was used to stain cells. Acetylcholinesterase (AChE) assay, transmission electron microscopy (TEM), and western blotting characterized Exosomes. Exosomal miR-21, miR-126, matrix metallopeptidase-9 (MMP-9), and tumor necrosis factor- ɑ (TNF-ɑ) proteins were measured by Q-PCR and western blotting. A wound-healing assay was used to explore the effect of exosomes on ECs migration rate. The results showed that the expression of P53, P21, P16, FMR1, and miR-21 was increased in treated cells as compared with control cells (P < 0.05). In addition, the expression of miR-126 was decreased in treated cells (P < 0.05). The number of Oil Red O-positive-treated cells increased (P < 0.05). The AChE activity of exosomes from treated cells was increased (P < 0.05). In comparison with control cells, an increase in the expression levels of exosomal miR-21 and TNF-ɑ of treated cells coincided with a decrease in the expression levels of miR-126 and MMP-9 levels (P < 0.05). We found that the migration rate of ECs co-cultured with exosomes from treated cells was decreased (P < 0.05). The data indicate ECs under H 2 O 2 condition produce exosomes with distinct cargo that may be useful as a biomarker of age-related vascular disease
Item Type: | Article |
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Uncontrolled Keywords: | Endothelial senescence · miR-21 · Exosomes · H2O2 · P53 · P21 |
Subjects: | R Medicine > R Medicine (General) |
Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
Date Deposited: | 05 Nov 2022 08:10 |
Last Modified: | 05 Nov 2022 08:10 |
URI: | http://eprints.umsu.ac.ir/id/eprint/6463 |
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