Intra‑tracheal delivery of mesenchymal stem cell‑conditioned medium ameliorates pathological changes by inhibiting apoptosis in asthmatic rats

Keyhanmanesh, Rana and Khodamoradi, Fatemeh and Rahbarghazi, Reza and Rahbarghazi, Afshin and Rezaie, Jafar and Ahmadi, Mahdi and Salimi, Leila and Delkhosh, Aref (2022) Intra‑tracheal delivery of mesenchymal stem cell‑conditioned medium ameliorates pathological changes by inhibiting apoptosis in asthmatic rats. Molecular Biology Reports.

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Abstract

Asthma, an inflammatory illness of the lungs, remains the most common long-term disease amongst children. This study tried to elaborate the status of apoptosis in asthmatic pulmonary niche after the application of rat mesenchymal stem cells (MSC-CM)-derived secretome. Methods and results Here, we randomly allocated male Wistar rats into three groups (n = 8); Control animals were intratra- cheally given 50 μl vehicle. In control-matched sensitized rats, 50 μl normal saline was used. In the last group, 50 μl MSC- CM was applied. Two-week post-administration, transcription of T-bet, GATA-3, Bax, Bcl-2 and Caspase-3 was measured by gene expression analysis. Pathological injuries were monitored using H&E staining. The BALF level of TNF-α was measured using ELISA assay. In asthmatic rats received MSC-CM, the expression of T-bet was increased while the level of GATA-3 decreased compared to the S group (p < 0.05). Levels of BALF TNF-α were suppressed in asthmatic niche after MSC-CM administration (p < 0.05). Compared to the asthmatic group, MSC-CM had potential to alter the expression of apoptosis-related genes in which the expression of Bax and Caspase 3 was decreased and the expression of pro-survival factor, Bcl-2 increased (p < 0.05). Conclusion Our data notified the potency of direct administration of MSC-CM in the alleviation of airway inflammation, presumably by down regulating apoptotic death in pulmonary niche

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