Design and evaluation of a multi-epitope vaccine for COVID- 19: an in silico approach

Tourani, Mehdi and Samavarchi Tehrani, Sadra and Movahedpour, Ahmad and Rezaei Arablouydareh, Sahar Rezaei and Maleksabet, Amir and Savardashtaki, Amir and Ghasemnejad‐Berenji, Hojat and Taheri-Anganeh, Mortaza (2023) Design and evaluation of a multi-epitope vaccine for COVID- 19: an in silico approach. Health Science Monitor, 2 (3).

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Background & Aims: Corona virus disease-19 (COVID-19) is an evolving global disease which has burst into 2019. SARS-CoV-2 infects human cells through recognition of and binding to angiotensin converting enzyme-2 (ACE2) through the spike (S) glycoprotein. Spike is an immunogenic protein that can elicit immune responses. Multi-epitope vaccines are novel and efficient class of vaccines which are designed by linking the B and T cells. These epitopes stimulate both humoral and cellular immunity. Materials & Methods: Based on bioinformatics online tools, appropriate epitopes of S protein were selected, linked together via suitable linkers, a TLR4 binding adjuvant was added, and a multi-epitope construct was constructed. The 3D model of the construct was predicted, refined, and validated. The antigenicity, allergenicity, solubility, and physico-chemical properties of vaccine were checked. The B cell conformational epitopes and IFN-γ inducing parts were detected. The adjuvant and TLR4 binding were evaluated by docking and protein-protein complex stability was assessed by elastic- mode analysis. The coding sequence of the vaccine construct was optimized and sub-cloned in expression vector through an in silico approach. Finally, the structure, energy, and stability of vaccine coding mRNA were evaluated. Results: Ten continuous B cell epitopes, 9 T helper epitopes, and 8 CTL epitopes were chosen. The results showed that multi-epitope vaccine is a stable and soluble protein which can stimulate humoral and cellular immunity. Besides, the vaccine could stimulate immunity without inducing allergenicity in human body. Conclusion: Finally, the vaccine can bind the TLR4 appropriately and can be expressed by a recombinant vector. The designed multi-epitope vaccine against COVID-19 could be considered as a suitable candidate for experimental studie

Item Type: Article
Uncontrolled Keywords: Bioinformatics, COVID-19, SARS-CoV-2, Spike glycoprotein, Vaccine
Subjects: R Medicine > R Medicine (General)
Depositing User: Unnamed user with email
Date Deposited: 18 Nov 2023 09:15
Last Modified: 18 Nov 2023 09:15

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