Soleimanzadeh, A and Saberivand, A and Ahmadi, A (2014) EFFECT OF Α-TOCOPHEROL ON SPERMATOZOA OF RAT SEMEN AFTER THE FREEZE-THAWING PROCESS. The Journal of Urmia University of Medical Sciences, 25 (9). pp. 826-834.
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Abstract
Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. -tocopherol is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the antioxidant effects of - tocopherol on count, motility, viability, TAC capacity, and DNA integrity rat spermatozoa during semen freezing process. Materials & Methods: Collected sperm from 10 adult rats were divided into 3 groups: fresh sperm, control and -tocopherol (200 μM). After freezing-thawing, the number of spermatozoa, motility, viability, TAC capacity of the sperm was analyzed. DNA integrity was assessed by nuclear staining using acridine orange. Results: After freezing-thawing, there was a significant increase in the motility, viability and sperm DNA integrity in -tocopherol group compared to the control (p<0.05). Following cryopreservation, no significant differences were found in morphology and sperm count between groups. Conclusion: Based on our results, it is concluded that addition of -tocopherol during freezing resulted in positive effects on sperm parameters after thawing in adult rats.
| Item Type: | Article |
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| Uncontrolled Keywords: | -tocopherol, Sperm parameters, DNA integrity, Freeze, Thawing, Rat |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
| Date Deposited: | 29 Oct 2017 05:36 |
| Last Modified: | 03 Jul 2019 05:29 |
| URI: | http://eprints.umsu.ac.ir/id/eprint/3180 |
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