INVESTIGATING THE EFFECTS OF LOW FREQUENCY ELECTROMAGNETIC FIELD ON MCF-7 CANCER CELL LINE

Sadooghi, S.D and Balanezhad, S.S and Nezhad Shahrokh Abadi, KH and Baharara, J (2014) INVESTIGATING THE EFFECTS OF LOW FREQUENCY ELECTROMAGNETIC FIELD ON MCF-7 CANCER CELL LINE. The Journal of Urmia University of Medical Sciences, 25 (5). pp. 444-452.

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Abstract

Breast cancer is the most common cancer in women. The aim of this study was to investigate the effects of electromagnetic field on MCF-7 cancer cell line. Materials & Methods: MCF-7 cell lines and L929 cell line were cultured in RPMI medium that contain fetal bovine serum and antibiotics. Cells for three hours were exposed to 50 Hz electromagnetic field with intensities of 50, 200 and 400 Gauss. Growth and proliferation and morphological changes were photographed by inverted microscope. Cell viability was quantitated by MTT assay. The obtained data were statistically analyzed by SPSS software and compared with each other by ANOVA and post hoc Tukey. Results: Viability percent of MCF-7 (87.985±5.38) and L929 (93.726±4.28) cell line that were vicinity of electromagnetic field with intensity of 50 gauss was not significantly compared to control. Viability percent of MCF-7 (41.342±8.27) and L929 (44.649±3.39) cell line that were vicinity of electromagnetic field with intensity of 200 gauss was significant compared to control. Viability percent of MCF-7 (24.381±5.98) and L929 (30.627±7.55) cell line that were vicinity of electromagnetic field with intensity of 400 gauss was significant compared to control. Conclusion: Electromagnetic field with frequency of 50 Hz and intensities of 200 and 400 Gauss was inhibitory effect on the growth and proliferation of MCF-7 and L929 cell line

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