Genotyping the human papilloma virus infection in Iranian women referred to ShahidMotahari Hospital, in Urmia, with Real-Time PCR techniques

Bagheri Hamzyan Olia, J and Khadem Ansari, M.H and Yagmaei, P and Khalkhali, H.R and Ayatollahi, H (2018) Genotyping the human papilloma virus infection in Iranian women referred to ShahidMotahari Hospital, in Urmia, with Real-Time PCR techniques. International Journal of Research in Applied and Basic Medical Sciences, 4 (2). pp. 60-67.

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Abstract

Human papilloma virus is inviridae of papilloma virus (HPV) family. These viruses have been classified based on the DNA sequences. At least 120 types of these viruses have been identified. Different types of human papilloma virus genotypes are associated with lower genital tract infection. The viruses in the host cell can create lethal, chronic, latent and transforming infections, and their multiple genotypes are related to human cancers such as cervical cancer.This study aimed to identify the genotypes of human papilloma virus that cause vaginal infections in women with infection. Materials and Methods: This cross-sectional study was performed on 80 patients with vaginal infection who referred to specialized gynecology unit of Kosar Health Center of ShahidMotahari Hospital of Urmia University of Medical Sciences (Iran). Extracting viral DNA was performed automatically using Mag Core Nucleic Acid Extractor (made in Taiwan) and MagCor®viral nucleic Acid Extration kit (Cartridge code 202) made by MogCore Co. Taiwan, and genotyping the samples was carried out using the Real-Time PCR technique in the Lightcycler 96 system (made in Germany), and Real quality RQ-HPV HR/LR Multiplex Kit made by AB Analitica® Co. Italy. Results: Among the 80 samples, 30 people (37.5%) were positive for infection with human papillomavirus, evaluating the positive genotypes, it was found that of these, 16 people (53.4%) were infected with Low-Risk genotypes of human papilloma virus (6, 11, 26, 53, 67),7 patients (23.3%) were infected with only High-Risk HPV genotype (16, 31, 58, 18) and 7 people (23.3%) were infected with both low-risk and high-risk genotypes of HPV (co-infection) (6, 33, 53, 39, 68, 70, 52, 35, 26, 51, 16), respectively. Conclusion: Considering that human papilloma virus infection is asymptomatic, and it has a high prevalence in Iran, according to studies, early diagnosis, and prevention of progressing the infection can prevent the malignancies of the uterus.Molecular techniques, particularly Real-time PCR, are as one of the fast and reliable methods for detecting the human papilloma virus infections even with quite low viral loads.

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