Evaluation the Anti-proliferative Effect of NVP-AUY922 in Combination with Thymoquinone in Colorectal Cancer Cell Line

Toofani milani, A and Paribanaem, R and Mohammadian, M (2021) Evaluation the Anti-proliferative Effect of NVP-AUY922 in Combination with Thymoquinone in Colorectal Cancer Cell Line. Journal of Research in Applied and Basic Medical Sciences, 7 (3). pp. 122-127.

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Abstract

Thymoquinone (TQ) is a natural component and the active herbal complex originate in Nigella sativa seed. TQ shows the anti-cancer effects in the previous studies. The effects of TQ, its mechanism on colorectal cancer, and its combination with other newly chemotherapeutic agents are unclear. Heat shock protein 90 (HSP90) has been upregulated in the numbers of malignancies. In this survey, we investigated the impacts of TQ and NVP-AUY922 (a HSP90 inhibitor) on HT-29 colorectal cancer cell line. Materials & Methods: HT-29 cells were seeded and exposed to TQ and NVP-AUY922 for 24 hours in various concentrations. Cell viability (water-soluble tetrazolium-1) assay was performed. Moreover, in combination cases, various concentrations of both agents examined using cellular viability analysis. Results: The TQ significantly inhibited cancer cell growth in colorectal cancer cell line in combination with various concentration of NVP-AUY922. Treatment with TQ could augment the cytotoxicity of NVP-AUY922 against the HT-29 as compared with that of NVPAUY922 alone. Conclusion: Our findings suggested the anti-proliferative effect of TQ and NVP-AUY922 through cytotoxic pathway to induce cell death.

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