THE EFFECT OF CONTINUOUS TRAINING ON RATE OF CASPASE3, BAX AND BCL-2 PROTEINS IN VASCULAR OF INDUCED POISONING INDUCED BY DIFFERENT DOSES OF HYDROGEN PEROXIDE

Apoptosis is organized cellular death, induced by various physiological and
pathological factors. Therefore, the purpose of this study is to investigate the effect of continuous training on
rate of Caspase3, BAX and BCL-2 proteins in rats vascular by induced poisoning due to various doses of
hydrogen peroxide.
Materials & Methods: 40 mature male wistar rats were randomly assigned into 5 groups including 8 rats in
each group. The groups were injected by: 1-Intraperitoneal injection of 1mM H2O2, group (H1), 2-
Intraperitoneal injection of 2mM H2O2, group(H2), 3-Intraperitoneal injection of 1mM H2O2 with regular
exercise, group (H1E), 4- Intraperitoneal injection of 2mM H2O2 with regular exercise, group (H2E), and 5-
control group(C).The training groups performed regular training on the treadmill daily for 8 weeks, all of rats
after the anaesthesia knocked out and the BAX, BCL-2 and Caspas3 proteins ratio were determined and
measured by ELISA technic. The ANOVA Two Way test was used to determine the effect of each of the
independent variables on the indicators. Also, the ANOVA One Way test of the Bonfroni follow-up test was
used to examine the intergroup of each of the variables. And significance was considered as P≤ 00.05.
Results: Continuous training in the rats showed that the amount of BAX protein did not decrease significantly
compared to the treatment group with 1 and 2 mM of hydrogen peroxide. Exercise alone or with injections
of 1 and 2 mM of hydrogen peroxide, increased the amount of protein BCL-2, which was not significant and
decreased Caspase3, BAX, and BAX / BCL-2 ratios in both groups receiving one and two milliliters of
hydrogen peroxide, but only changes in Caspase-3 (P = 0.014) and ratio BAX / BCL-2 (p = 0.0002) was
significant in groups receiving 2 mM of hydrogen peroxide (P ≤ 0.05).
Conclusion:Continuous exercises are likely to reduce the apoptosis of endothelial cells by reducing
Caspase3, BAX / BCL-2 ratio and increasing BCL-2 which is likely to prevent vascular tissu damage.