INVESTIGATING THE EFFECT OF ELECTROMAGNETIC FIELD WITH LOW FREQUENCY ON DEVELOPMENT OF TWO-CELL NMRI MICE EMBRYO (IN VITRO)

Rahbarian, R and Sadooghi, S.D (2014) INVESTIGATING THE EFFECT OF ELECTROMAGNETIC FIELD WITH LOW FREQUENCY ON DEVELOPMENT OF TWO-CELL NMRI MICE EMBRYO (IN VITRO). The Journal of Urmia University of Medical Sciences, 25 (1). pp. 67-75.

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Abstract

With increase in electrical devices, pregnant women has been exposed to electromagnetic field (EMF) radiation with low-frequency (50-60 Hz). The aim of this study is the investigating the effect of EMF with low frequency on development of two-cell NMRI mice embryo. Materials & Methods: Ovulation in female mice was induced by intraperitoneal injection of 10 IU PMSG. 48 hours later, 10 IU HCG was injected intraperitoneally. Then female mice were caged with male rats. Mice with vaginal plug were killed 48 hours after HCG injection and two-cell embryos in sterile conditions were collected in M16 medium. Embryos were divided into the control group (Incubated for 5 days), shame-exposed group (90 minutes in vicinity of switched off EMF generating system) and six experimental groups that were directly exposed to low frequency EMF with intensities of 10 and 200 gauss for 30, 60 and 90 minutes. In all groups, percentage of embryonic development were examined on days 1, 2, 3, 4 and 5 of experiment. The obtained data was statistically analyzed by ANOVA and post hoc Tukey (p<0.05). Results: Number and percentage of embryonic development of group that was daily (5 consecutive days) exposure to EMF with intensity of 10 gauss for 90 minutes, compare with the control group significantly decreased in each of the experiment days (p<0.05). Number and percentage of embryonic development of groups that were daily (5 consecutive days) exposure to EMF with intensity of 200 gauss for 30, 60 and 90 minutes, compare with the control group significantly decreased in each of the experiment days (p<0.05). Conclusion: Low frequency electromagnetic field with high intensity can be reduced and stop embryonic development in mice.

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