INVESTIGATING THE EFFECT OF ELECTROMAGNETIC FIELD WITH LOW FREQUENCY ON DEVELOPMENT OF TWO-CELL NMRI MICE EMBRYO (IN VITRO)

With increase in electrical devices, pregnant women has been exposed to
electromagnetic field (EMF) radiation with low-frequency (50-60 Hz). The aim of this study is the
investigating the effect of EMF with low frequency on development of two-cell NMRI mice embryo.
Materials & Methods: Ovulation in female mice was induced by intraperitoneal injection of 10 IU
PMSG. 48 hours later, 10 IU HCG was injected intraperitoneally. Then female mice were caged with
male rats. Mice with vaginal plug were killed 48 hours after HCG injection and two-cell embryos in
sterile conditions were collected in M16 medium. Embryos were divided into the control group
(Incubated for 5 days), shame-exposed group (90 minutes in vicinity of switched off EMF generating
system) and six experimental groups that were directly exposed to low frequency EMF with intensities
of 10 and 200 gauss for 30, 60 and 90 minutes. In all groups, percentage of embryonic development
were examined on days 1, 2, 3, 4 and 5 of experiment. The obtained data was statistically analyzed by
ANOVA and post hoc Tukey (p<0.05).
Results: Number and percentage of embryonic development of group that was daily (5 consecutive
days) exposure to EMF with intensity of 10 gauss for 90 minutes, compare with the control group
significantly decreased in each of the experiment days (p<0.05). Number and percentage of embryonic
development of groups that were daily (5 consecutive days) exposure to EMF with intensity of 200
gauss for 30, 60 and 90 minutes, compare with the control group significantly decreased in each of the
experiment days (p<0.05).
Conclusion: Low frequency electromagnetic field with high intensity can be reduced and stop
embryonic development in mice.