PROTECTIVE EFFECT OF NANO ZINC OXIDE ON TESTIS AND SEX HORMONE LEVELS OF MICE AFTER TREATMENT WITH PHENYLHYDRAZINE

Phenylhydrazine cause adverse effect on testis tissue and hormone levels. This
study was conducted to evaluate the effects of nano zinc oxide on the Testis Tissue and sex hormone
levels after Phenylhydrazine-induced hypoxia in male mice.
Materials & Methods: In order to perform the study 72 adult male mice with the capability of fertilizing
with the age range of 6-8weeks randomly divide to 9 groups. Group 1 (control); Group 2
(Phenylhydrazine 8 mg/100 gr/b.w/ i.p. first time and 6 mg/100 gr/b.w/ i.p. every 48 hour); Group 3
(Sham 0.1 ml/kg/ i.p.), Group 4 (Phenylhydrazine + nano zinc oxide 1 mg/kg/i.p.); Group 5
(Phenylhydrazine + nano zinc oxide 2.5 mg/kg/i.p.); Group 6 (Phenylhydrazine + nano zinc oxide 5
mg/kg/i.p.); Group 7 (nano zinc oxide 1 mg/kg/i.p.); Group 8 (nano zinc oxide 2.5 mg/kg/i.p.) and Group
9 (nano zinc oxide 5 mg/kg/i.p.). 35 days after the last treatment, they were euthanized and following
analyzing the sperm features, morphologic and mophometric changes, serum level of testosterone and
LH and FSH levels were also evaluated. Data were analyzed using SPSS and a value of p < 0.05 was
considered as statistically significant.
Results: The groups receiving only Phenylhydrazine showed a considerable reduction in testicles
weight, spermatogenesis indicies, Tubular Differentiation Index, Spermatogenic Index and, most of the
semniferous tube laked spermatozoa and the height epithelial and number of sertoli cell and leydig cell
and diameter of semniferous tube had decreased, and the interstitial tissue increased and edmatos.
However treatment with nano zinc oxide caused considerable improvement in mentioned parameters.
Conclusion: The results of this study showed that nano zinc oxide in lower dose (2.5 mg/kg) can have
protective role against adverse effect caused by phenylhyrazine