Evaluation of anti-proliferative effects of Crocus pallasii subsp. Haussknechtii on MCF-7 cells

Naji, S and Nakhostinfar, N and Ebrahimpour, M and Zarei, L and Mehrshad, A (2017) Evaluation of anti-proliferative effects of Crocus pallasii subsp. Haussknechtii on MCF-7 cells. International Journal of Advanced Biotechnology and Research (IJBR), 8 (3). pp. 2394-2400.

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Abstract

Breast cancer is the most common cancer and is the second leading cause of death among women. It is necessary to pay attention to the role of natural antioxidant compounds along with chemotherapy that can prevent or reduce the rate of progression of cancer and the complications of synthetic drugs. Species of the Crocus genus has been proved to have anti-proliferative and apoptosis inducing properties against various cancerous cells. The purpose of this study was to evaluate the anti-proliferative effects of Crocus pallasii subsp. Haussknechtii on MCf-7 cells as a natural product with fewer side effects. Materials and Methods: After cell culture, treatment was performed with the extract of 0.15, 0.20 and 0.25 mg / ml, and 0.1 doses of doxorubicin as a positive control for 24 hours. Then the cells proliferation and viability of cells were measured using the MTT assay and staining of acridine orange-propidium iodide by a spectrophotometer and fluorescence microscope, respectively. Results: After analyzing the data, it was observed that the amount of proliferation and survival in the cancerous cells treated with Crocus pallasii subsp. Haussknechtii was significantly reduced compared to the normal group. Conclusion: It seems that the extract had less anti-proliferative effects compared to doxorubicin, but the extract could be considered as a natural remedy

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