Evaluation of anti-proliferative effects of Crocus pallasii subsp. Haussknechtii on MCF-7 cells

Breast cancer is the most common cancer and is the second leading cause of death among women. It is
necessary to pay attention to the role of natural antioxidant compounds along with chemotherapy that can prevent or
reduce the rate of progression of cancer and the complications of synthetic drugs. Species of the Crocus genus has
been proved to have anti-proliferative and apoptosis inducing properties against various cancerous cells. The purpose
of this study was to evaluate the anti-proliferative effects of Crocus pallasii subsp. Haussknechtii on MCf-7 cells as a
natural product with fewer side effects.
Materials and Methods: After cell culture, treatment was performed with the extract of 0.15, 0.20 and 0.25 mg / ml,
and 0.1 doses of doxorubicin as a positive control for 24 hours. Then the cells proliferation and viability of cells were
measured using the MTT assay and staining of acridine orange-propidium iodide by a spectrophotometer and
fluorescence microscope, respectively.
Results: After analyzing the data, it was observed that the amount of proliferation and survival in the cancerous cells
treated with Crocus pallasii subsp. Haussknechtii was significantly reduced compared to the normal group.
Conclusion: It seems that the extract had less anti-proliferative effects compared to doxorubicin, but the extract could
be considered as a natural remedy