PREVALENCE OF HELICOBACTER PYLORI BABA2, BABB AND OIPA GENOTYPES IN DYSPEPTIC PATIENTS

Milani, M and Akbarzadeh, A and Somi, M (2018) PREVALENCE OF HELICOBACTER PYLORI BABA2, BABB AND OIPA GENOTYPES IN DYSPEPTIC PATIENTS. The Journal of Urmia University of Medical Sciences, 29 (4). pp. 255-263.

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Abstract

Helicobacter pylori is a microaerophilic gram negative bacillus which is one of the most common human infections. This bacterium is associated with various diseases and having multiple virulence genes such as toxins and adhesion factors. Several adhesion factors of bacteria involved in binding to the gastric epithelial cell and cause infection. The purposes of this study were to assess the babA2, babB and oipA genes to H. pylori isolated from Imam Reza Hospital of Tabriz. Materials & Methods: Biopsy specimens obtained from 340 patients (During the year 2016) were cultured on Brucella agar and 130 H. pylori isolates were collected. Genomic DNA of total H. pylori isolates was extracted by using CTAB method. PCR reaction was performed on glmM gene for the confirmation of H. pylori. The presence of the babA2, babB, oipA genes were determined by PCR method. Results: The prevalence of babA2, babB and oipA genes was positive in 62.3%, 73.8% and 54.6%, respectively. The endoscopic observations indicated 21 PUD and 109 NUD cases of total 130 isolations. These findings showed that the prevalence of the babA2, babB and oipA genes in PUD patients were 13.1%, 10.8% and 6.9%, respectively. Conclusion: Our findings showed that the prevalence of all three genes of babA2, babB and oipA in NUD patients is more than PUD patients. The oipA gene has a significant relation in developed clinical outcomes

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