PREVALENCE OF HELICOBACTER PYLORI BABA2, BABB AND OIPA GENOTYPES IN DYSPEPTIC PATIENTS

Helicobacter pylori is a microaerophilic gram negative bacillus which is one of
the most common human infections. This bacterium is associated with various diseases and having
multiple virulence genes such as toxins and adhesion factors. Several adhesion factors of bacteria
involved in binding to the gastric epithelial cell and cause infection. The purposes of this study were to
assess the babA2, babB and oipA genes to H. pylori isolated from Imam Reza Hospital of Tabriz.
Materials & Methods: Biopsy specimens obtained from 340 patients (During the year 2016) were
cultured on Brucella agar and 130 H. pylori isolates were collected. Genomic DNA of total H. pylori
isolates was extracted by using CTAB method. PCR reaction was performed on glmM gene for the
confirmation of H. pylori. The presence of the babA2, babB, oipA genes were determined by PCR
method.
Results: The prevalence of babA2, babB and oipA genes was positive in 62.3%, 73.8% and 54.6%,
respectively. The endoscopic observations indicated 21 PUD and 109 NUD cases of total 130 isolations.
These findings showed that the prevalence of the babA2, babB and oipA genes in PUD patients were
13.1%, 10.8% and 6.9%, respectively.
Conclusion: Our findings showed that the prevalence of all three genes of babA2, babB and oipA in
NUD patients is more than PUD patients. The oipA gene has a significant relation in developed clinical
outcomes