Nasiri Bari, Y and Zendehdel Khaybari, M and Akbari, G and Ahmadi, A and Babapour, W (2020) EVALUATION OF THE EFFECT OF HYPOTAURINE ON IN VITRO FERTILIZATION IN THE OOCYTES OBTAINED OF THE EXPERIMENTAL POLYCYSTIC OVARY SYNDROME IN MICE: AN EXPERIMENTAL STUDY. Studies in Medical Sciences, 31 (5).
|
Text
4 Nasiri Bari A-10-4251-1.pdf Download (2MB) | Preview |
Abstract
In this experimental study, the effect of hypotaurine on in vitro fertilization and fertility of oocytes from mice with experimental polycystic ovary syndrome was designed and performed. Materials & Method: In this experimental study, NMRI mice aged 6 to 8 weeks were used. The mice were divided into two groups, control and experimental PCOS. For induction of experimental PCOS, Estradiol Valerate (100 mg/kg, IP) was injected. Then, for ovulation, the injection was performed first with 7.5 IU PMSG in the volume of 0.1 ml and 46-48 hours later with 7.5 IU hCG in the volume of 0.1 ml. Finally, 0/1, 1 and 10 μM curcumin were added to the culture medium of oocytes of PCOS group and development in different groups were evaluated (P <0.05). Results: Most of the arrested embryos had a high percentage of lysis and fragmentation and the arrested embryos were type I and II. The addition of hypotaurin reduced the rate of lysis and fragmentation and decreased the percentage of arrested embryos. Lesser arrested embryos were type III (P <0.05). This study showed that 1 μM hypotaurin increased fertilization percentage and 10 μM hypotaurin increased the percentage of two-cell embryos and also increased the percentage of blastocytes (P <0.05). Conclusion: The results showed that addition of certain concentrations of hypotaurin as an antioxidant, improved and increased the fertilization rate and the percentage of two-cell embryos. It can also be helpful in increasing the percentage of blastocysts
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Polycystic Ovary Syndrome, Oocyte, In Vitro Fertilization, Hypotaurin, Mice |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
| Date Deposited: | 27 Sep 2020 07:51 |
| Last Modified: | 27 Sep 2020 07:51 |
| URI: | http://eprints.umsu.ac.ir/id/eprint/6018 |
Actions (login required)
 |
View Item |
