EVALUATION OF THE EFFECT OF HYPOTAURINE ON IN VITRO FERTILIZATION IN THE OOCYTES OBTAINED OF THE EXPERIMENTAL POLYCYSTIC OVARY SYNDROME IN MICE: AN EXPERIMENTAL STUDY

In this experimental study, the effect of hypotaurine on in vitro fertilization and
fertility of oocytes from mice with experimental polycystic ovary syndrome was designed and
performed.
Materials & Method: In this experimental study, NMRI mice aged 6 to 8 weeks were used. The mice
were divided into two groups, control and experimental PCOS. For induction of experimental PCOS,
Estradiol Valerate (100 mg/kg, IP) was injected. Then, for ovulation, the injection was performed first
with 7.5 IU PMSG in the volume of 0.1 ml and 46-48 hours later with 7.5 IU hCG in the volume of 0.1
ml. Finally, 0/1, 1 and 10 μM curcumin were added to the culture medium of oocytes of PCOS group
and development in different groups were evaluated (P <0.05).
Results: Most of the arrested embryos had a high percentage of lysis and fragmentation and the arrested
embryos were type I and II. The addition of hypotaurin reduced the rate of lysis and fragmentation and
decreased the percentage of arrested embryos. Lesser arrested embryos were type III (P <0.05). This
study showed that 1 μM hypotaurin increased fertilization percentage and 10 μM hypotaurin increased
the percentage of two-cell embryos and also increased the percentage of blastocytes (P <0.05).
Conclusion: The results showed that addition of certain concentrations of hypotaurin as an antioxidant,
improved and increased the fertilization rate and the percentage of two-cell embryos. It can also be
helpful in increasing the percentage of blastocysts