Maleki, M and Abdolmaleki, A and Dezfoulian, M and Ghoreishi, S.A (2021) DIFFERENTIATION OF HUMAN OVARIAN FOLLICULAR GRANULOSA CELLS INTO KERATINOCYTES. Studies in Medical Sciences, 31 (11). pp. 813-822.
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Abstract
Stem cells are undifferentiated cells and are found in different tissues. These cells have capacity of self-renewal and differentiation into other lineages. Granulosa cells (GCs) are the multipotent stem cells. In the present research we evaluated the differentiation potential of GCs into keratinocytes. Material & Methods: GCs were cultured after enzymatic isolation from ovarian follicle. Then, keratinocyte inductive medium was added and expression of keratin10 and keratin14 were investigated with western blotting technique. Results: The results of the flow cytometric analysis of the isolated cells indicated the high expression of mesenchymal stem cell specific antigens (p < 0.05). Also, the results of the western blotting showed the expression of creatine 10 and creatine 14 proteins in all groups except for negative control (p< 0.05). Conclusion: Human granulosa cells have a very high ability to differentiate into keratinocytic cells, and with further research, it is possible to provide a suitable substrate for the use of human granulosa cells to treat severe skin lesions
| Item Type: | Article |
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| Uncontrolled Keywords: | Keratinocyte, Western blotting, Flow cytometry, Keratin10, Keratin14, Granulosa cells |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
| Date Deposited: | 23 Feb 2021 07:59 |
| Last Modified: | 23 Feb 2021 07:59 |
| URI: | http://eprints.umsu.ac.ir/id/eprint/6140 |
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