DIFFERENTIATION OF HUMAN OVARIAN FOLLICULAR GRANULOSA CELLS INTO KERATINOCYTES

Stem cells are undifferentiated cells and are found in different tissues. These cells
have capacity of self-renewal and differentiation into other lineages. Granulosa cells (GCs) are the
multipotent stem cells. In the present research we evaluated the differentiation potential of GCs into
keratinocytes.
Material & Methods: GCs were cultured after enzymatic isolation from ovarian follicle. Then,
keratinocyte inductive medium was added and expression of keratin10 and keratin14 were investigated
with western blotting technique.
Results: The results of the flow cytometric analysis of the isolated cells indicated the high expression
of mesenchymal stem cell specific antigens (p < 0.05). Also, the results of the western blotting showed
the expression of creatine 10 and creatine 14 proteins in all groups except for negative control (p< 0.05).
Conclusion: Human granulosa cells have a very high ability to differentiate into keratinocytic cells, and
with further research, it is possible to provide a suitable substrate for the use of human granulosa cells
to treat severe skin lesions