Nourooz Zadeh, J (2009) An improved procedure for the enrichment of plasma F2- isoprostanes prior to final determination by GC-MS/NICI. Scientia Iranica, 16 (2 C). pp. 140-144.
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Abstract
One of the most popular approaches to quantify oxidative injury is to measure lipid peroxidation products and, in particular, F2-isoprostanes (F2-IPs). F2-IPs is a group of prostaglandin F2-like compounds derived from the non-enzymatic oxidation of arachidonic acid. Of these, the 15-F2t-isoprostane (8-iso-PGF2�) has received considerable attention, because it possesses adverse biological activities. Previous Gas Chromatographic-Mass Spectrometric (GC-MS) methods for measuring plasma F2-IPs from this laboratory involved two chromatography steps on C18 and NH2-cartridges. Problems may, however, arise with chromatography on C18 cartridges, as it can be time-consuming and losses may occur depending upon the pH and e�ciency of the sample loading. Therefore, it was decided that the C18 chromatography step be replaced with a single lipid partitioning step and the NH2-chromatography be simpli�ed. In 70 plasma samples from healthy individuals, total (sum of free and esteri�ed) 15-F2t-isoprostane concentrations ranged from 0.5 to 3.13 nM. This assay meets all prede�ned method performances in terms of speci�city and sensitivity. The improved method is suitable for the analysis of samples from larger clinical trials investigating the role of oxidant injury under conditions associated with oxidative stress.
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 0 |
| Uncontrolled Keywords: | F2-isoprostanes; Lipid peroxidation; Oxidative stress; Gas chromatography-mass spectrometry. |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
| Date Deposited: | 16 Aug 2017 04:42 |
| Last Modified: | 01 Sep 2019 06:59 |
| URI: | http://eprints.umsu.ac.ir/id/eprint/1294 |
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