INVESTIGATING RESPONSE OF SPLEEN AND ERYTHROCYTES ANTIOXIDANT DEFENSE SYSTEM ON THE EFFECTS OF NACETYL CYSTEINE AGAINST PARAOXON TOXICITY IN RAT

Khazaei, S and Jafari, M and Heydari, J and Salem, F (2015) INVESTIGATING RESPONSE OF SPLEEN AND ERYTHROCYTES ANTIOXIDANT DEFENSE SYSTEM ON THE EFFECTS OF NACETYL CYSTEINE AGAINST PARAOXON TOXICITY IN RAT. The Journal of Urmia University of Medical Sciences, 26 (3). pp. 176-184.

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Abstract

Paraoxon (POX) as an organophosphate pesticide is the active form of parathion that is widely used in agriculture. Antioxidants can protect cells from oxidative stress. The aim of this study was to investigate the effect of NAC as an antioxidant against POX- induced oxidative stress in rat spleen and erythrocytes. Material & Methods: In the present experimental study, male Wistar rats were randomly divided into four groups including: control group (corn oil as POX solvent), POX group (0.7 mg/kg), NAC group (160 mg/kg), and NAC+POX all of which were given intraperitoneally. Then, 24 hours after injection, the animals were anesthetized, spleen tissues were quickly removed and blood was also collected by cardiac puncture; and erythrocytes were obtained. Then, superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods. Results: POX increased SOD and GST activities and decreased GSH content in rat spleen and erythrocytes. Also, POX increased CAT activity and MDA level in erythrocytes and decreased CAT activity in spleen. Administration of NAC inhibited the changes in these parameters. Conclusion: POX with free radical production and depleted GSH content leads to oxidative stress induction in spleen and erythrocytes. Administration of NAC as antioxidant decreases POX-induced oxidative stress by scavenging free radicals and GSH synthesis, but it does not protect completely.

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