ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATED FROM URINE SPECIMENS OF RENAL TRANSPLANT PATIENTS AND DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF ISOLATES TO CEFOTAXIME

Kashef Nejad, M and Sharifi, Y and Hosseini Jazani, N and Babazadeh, H (2015) ANTIMICROBIAL SUSCEPTIBILITY PATTERN OF ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATED FROM URINE SPECIMENS OF RENAL TRANSPLANT PATIENTS AND DETERMINATION OF MINIMUM INHIBITORY CONCENTRATION (MIC) OF ISOLATES TO CEFOTAXIME. The Journal of Urmia University of Medical Sciences, 26 (5). pp. 400-409.

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Abstract

Urinary tract infection (UTI) is the most common bacterial infection in renal transplant recipients. The aim of this study was to determine antimicrobial susceptibility patterns of Escherichia coli and Klebsiella pneumoniae isolates as well as determining cefotaxime Minimum Inhibitory Concentration (MIC) for obtained isolates. Materials & Methods: Escherichia coli and Klebsiella pneumoniae were obtained and identified from urine samples of renal transplant patients, according to standard bacteriologic tests. The antimicrobial susceptibility of the isolates to cefotaxim, ceftazidim, nitrofurantoin, imipenem, ciprofloxacin, aztreonam, erthapenam, ampicillin, gentamicin, cotrimoxazole were investigated using the disc diffusion method and MIC of cefotaxim for each isolate was determined using E-test. Results: Of 96 isolates that were collected from urine specimens, 57 isolates were identified as E. coli (59.4%) and 39 as Klebsiella pnumoniae (40.6 %). Considering antibiotic susceptibility patterns of disc diffusion method, high resistance to ampicillin (95.8%) and trimethoprim-sulfamethoxazole (78.1 %) were observed, and the least levels of resistance was observed to imipenem (10.4 %). In total, 67.7 % of isolates were resistant to cefotaxime by determining MIC values. Conclusion: Accordingly, this study indicate the spread of multi-drug resistance isolates among the renal transplant patients in Urmia and it seems to be an obstacle in usage of cephalosporin’s especially cefotaxime in treatment of such infections and insists on continuous laboratory screening and control of such isolates.

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