Mohammadzadeh, A and Shahrokhi, S and Pourfathollah, A.A and Amari, A and Taher Tahoori, M and Fallah, A and Soleimani, M and Forouzandeh, M (2016) Evaluation of AD-MSC (adipose-derived mesenchymal stem cells) as a vehicle for IFN-β delivery in experimental autoimmune encephalomyelitis. Clinical Immunology, 169. pp. 98-106.
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Abstract
Interferon-β (IFN-β) is commonly used as a disease modifying drug for the treatment of relapse-remitting multiple sclerosis (RR-MS). However, the underlying mechanism by which IFN-β mediate this immunosuppressive effect is still unknown. In this study, we analyzed the effects of genetically modified adipose-derived mesenchymal stem cells (AD-MSCs) expressing murine interferon beta (MSCs-VP/IFN-β) on the animal model of MS, experimen tal autoimmune encephalomyelitis (EAE). Lymph node mononuclear cells and serum were examined by using RT-PCR and ELISA methods to measure the production of IL-10 and IL-17 gene and protein expression, respectively. Our results indicated that in the MSCs-VP/IFN-β treated group induction of Tregs and IL-10 and reduction of IL-17 were significant. Taken together, we showed that using AD-MSCs expressing IFN-β as an anti-inflammatory agent, offer evidence supporting that the stem cell therapies in EAE conceivably will improve the valuable effects of IFN-β in this autoimmune disease.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MSCs (mesenchymal stem cells) IFN-β Treg EAE |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
| Date Deposited: | 02 Oct 2018 06:37 |
| Last Modified: | 13 Feb 2019 05:37 |
| URI: | http://eprints.umsu.ac.ir/id/eprint/5141 |
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