Mohammad, R.Y and Somayyeh, G and Gholamreza, H and Majid, M and Yousef, R (2013) Diosgenin inhibits hTERT gene expression in the A549 lung cancer cell line. Asian Pacific Journal of Cancer Prevention, 14 (11). pp. 6945-6948.
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Abstract
Diosgenin, a steroidal saponin from a therapeutic herb, fenugreek (Trigonellafoenum-graceum L.), has been recognized to have anticancer properties. Telomerase activity is not detected in typical healthy cells, while in cancer cell telomerase expression is reactivated, therefore providing a promising cancer therapeutic target. Materials and Methods: We studied the inhibitory effect of diosgenin on human telomerase reverse transcriptase gene (hTERT) expression which is critical for telomerase activity. MTT- assays and qRT-PCR analysis were conducted to assess cytotoxicity and hTERT gene expression inhibition effects, respectively. Results: MTT results showed that IC50 values for 24, 48 and 72h after treatment were 47, 44 and 43μM, respectively. Culturing cells with diosgenin treatment caused down-regulation of hTERT expression. Discussion: These results show that diosgenin inhibits telomerase activity by down-regulation of hTERT gene expression in the A549 lung cancer cell line
| Item Type: | Article |
|---|---|
| Additional Information: | cited By 11 |
| Uncontrolled Keywords: | Lung cancer - hTERT - diosgenin - telomerase - qRT-PCR - MTT assay |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Unnamed user with email gholipour.s@umsu.ac.ir |
| Date Deposited: | 30 Jul 2017 04:21 |
| Last Modified: | 08 Jul 2019 04:24 |
| URI: | http://eprints.umsu.ac.ir/id/eprint/756 |
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