Identification of echinococcus granulosus strains in isolated hydatid cyst specimens from animals by PCR-RFLP method in West Azerbaijan - Iran

Hanifian, H and Diba, K and Hazrati Tappeh, KH and Mohammadzadeh, H and Mahmoudloo, R (2013) Identification of echinococcus granulosus strains in isolated hydatid cyst specimens from animals by PCR-RFLP method in West Azerbaijan - Iran. Iranian Journal of Parasitology, 8 (3). pp. 376-381.

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Abstract

The aim of this study was DNA extraction from protoscolecses of Echinococcus granulosus and identification of these strains in West- Azerbaijan Province, north western Iran. Methods: Thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. To investigate the genetic variation of the isolates, after DNA extraction by Glass beads-phenol chloroform method; PCR-RLFP analysis of rDNA-ITS1 was performed using three different restriction enzymes of Taq 1, Rsa 1 and Alu 1. Result: Amplified PCR products for all isolates were 1000bp band which is expected band in sheep strains (G1-G3 complex). The results of RFLP analysis also were the same for all isolates. PCR-RFLP patterns restriction enzymes were identical as follows, Rsa1 bands under UV showed two bands approximately 655bp and 345bp. Alu1 bands were as follows: two approximately 800bp and 200bp and Taq1 did not cut any region and bands were approximately 1000 bp in all samples. Conclusions: Based on PCR-RFLP patterns of ITS1 fragment produced with endonucleases enzyme digestion in animal isolates, it can be concluded that a single strain of E. granulosus (sheep strain or G1-G3 complex) is dominant genotype in this province

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