Effect of different concentrations of hypertonic saline at different times on protoscoleces of hydatid cyst isolated from liver and lung.

Most surgeons inject scoloidal materials into the cyst before or after its removal, since any contamination to normal sites will
cause re-growth of the same cyst. The aim of this study was to determine the lethal effect of hypertonic saline at different doses and different
times on protoscolexes of lung and liver.
Methods: The livers and lungs of killed animals with hydatid cyst disease were gathered from Urmia Industrial Abattoirs. They were transferred
to the university parasitological lab immediately. The hydatid cyst fluid was aspirated with a 10 mm syringe and poured into a 15cc
tubes. The movement of protoscoleces and staining with 0.1% eosin was the test to determine viability of protoscoleces. Those with color
absorption were those which were not viable. Different concentrations of hypertonic saline were given at different time.
Results: The results showed that in 20% of hypertonic saline in the 4th minute, 80% of protoscoleces were alive while in the 5th minute 50%
were alive, in the 7th minute 20% and 8th minute 5%, 9th minute all of them were dead. In the 10% concentration, at up to 9 minutes 50% were
alive, in the 18th minute 20% and in 30 minutes 10% of protoscoleces were alive. In the 5% concentration at up to 10 minutes 90% were alive
while in the 22nd minute 80% and in 30 minutes 70% of protoscoleces were alive.
Conclusion: When we inject 20% hypertonic saline into the cyst cavity there is aprobability that the cyst contaminates the bile duct and liver
through the small hole we made. This material may cause widespread necrosis of the liver. We should use 10% hypertonic saline minimally
for 45 minute before surgery and after cyst removal, since the hypertonic saline itself may cause injury to the biliary system