The effect of cerebrospinal fluid in collagen guide channel on sciatic nerve regeneration in rats

The aim of this study was to evaluate the effect of cerebrospinal fluid (CSF) in nerve regeneration across the collagen guide
channel in comparison with autograft.
Mater Ial and Methods: Forty adult male rats (250-300 g) were randomized into (1) collagen channel+CSF, (2) collagen
channel+normal saline (NS), (3) autograft, and (4) sham surgery groups. The left sciatic nerve was exposed and a 10 mm nerve
segment was cut and removed. In the collagen groups, the proximal and distal cuts ends of sciatic nerve were telescoped into the
nerve guides and CSF or NS injected into collagen conduit. In the autograft group, the 10 mm nerve segment was turned backwards
and used an autologous nerve graft. All animals were evaluated by sciatic functional index (SFI) and electrophysiology, histology,
and immunohistochemistry testing.
Results: The improvements in SFI since the beginning of the last evaluation in experimental groups were measured. On days 49
and 60 post-operation, the mean SFI of the collagen+CSF group was significantly greater than the autograft group (P< 0.05). On day
90, the mean nerve conduction velocity (NCV) of the collagen+CSF group was greater than autograft group (P< 0.05). The number
of myelinated fibers in the collagen+CSF group was significantly greater than that of the collagen + NS group at day 90 (P<0.05).
ConclusIon: CSF in collagen nerve guide channel effectively enhances nerve regeneration and promotes functional recovery in
injured sciatic nerve of rats.