Association of interleukin-10, interferon-gamma, transforming growth factor-beta, and tumor necrosis factor-alpha gene polymorphisms with long-term kidney allograft survival

Single nucleotide polymorphisms within promoter or
other regulatory sequences of cytokine genes mainly influence the
level of production and secretion of proteins. A large amount of
evidence has shown that cytokine gene variations alter graft survival
length after kidney transplantation. We studied the association of
gene polymorphisms in the interlekin-10 gene (IL10; -1082 G/A),
interferon-γ gene (IFNG; +874 T/A), transforming growth factor-β
gene (TGFB; +869 T/C), and tumor necrosis factor-α gene (TNFA;
-308 A/G) with kidney allograft survival.
Materials and Methods. The IL10 (-1082 G/A), IFNG (+874 T/A),
TGFB (+869 T/C), and TNFA (-308 A/G) genotypes were determined
in 32 kidney allograft recipients with graft rejection during the 1st
posttransplant year and 52 without rejection in 5 posttransplant
years, using allele-specific oligonucleotides-polymerase chain
reaction method.
Results. The IFNG +874 A/T genotype showed a significantly
higher frequency among kidney recipients of the rejection group
than the control group (odds ratio, 2.64, 95% confidence interval,
1.03 to 6.74; P = .04). Comparisons between the rejection and control
groups in IL10 (-1082 G/A), IFNG (+874 T/A), TGFB (+869 T/C),
and TNFA (-308 A/G) single nucleotide polymorphisms showed
no significant difference.
Conclusions. Based on the finding of this study, it seems
polymorphisms in the genes that regulate IL-10, IFN-γ, TGF-β,
and TNF-α cytokines do not play a major role in kidney allograft
survival, and other potential factors in this regard should be
considered.